基因名称(Gene Name)
UBE2M UBC12
组成
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
来源
Monoclonal, Rabbit,IgG
稀释比例
IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
背景
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008],
功能
Catalytic activity:ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.,Domain:Both the N-terminal docking peptide and the catalytic core domain must bind the uba3-nae1 complex simultaneously for optimal transfer of nedd8.,Function:Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase rbx1, but not rbx2, suggests that the rbx1-ube2m complex neddylates specific target proteins, such as cul1, cul2, cul3 and cul4. Involved in cell proliferation.,pathway:Protein modification; protein neddylation.,similarity:Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.,subunit:Interacts with UBA3 and RBX1.,
