基因名称(Gene Name)
MAP2K3;MAP2K6
蛋白名称
Serine protease HTRA2 mitochondrial
特异性
MKK3 (Phospho Ser189)/MKK6 (Phospho Ser207) Antibody detects endogenous levels of MKK3/MEK6 protein only when phosphorylated at S189/S207.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):IHrDV/VDsVA
组成
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
来源
Monoclonal, Rabbit,IgG
稀释比例
IHC 1:100-1:500; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
其他名称
AW212142 ; dual specificity mitogen activated protein kinase kinase 3 ; Dual specificity mitogen activated protein kinase kinase 6 ; Dual specificity mitogen-activated protein kinase kinase 3 ; MAP kinase kinase 3 ; MAP kinase kinase 6 ; map2k3 ; MAP2K6 ; MAPK ERK kinase 3 ; MAPK/ERK kinase 3 ; MAPK/ERK kinase 6 ; MAPKK 3 ; MAPKK 6 ; MAPKK3 ; MAPKK6 ; MEK 3 ; MEK 6 ; MEK3 ; Mitogen activated protein kinase kinase 3 ; Mitogen activated protein kinase kinase 6 ; MKK 3 ; MKK3 ; MKK6 ; mMKK3b ; MP2K3_HUMAN ; PRKMK 3 ; PRKMK3 ; PRKMK6 ; Protein kinase, mitogen activated, kinase 6 ; MAP kinase kinase 6 ; protein kinase, mitogen-activated, kinase 3 ; SAPK kinase 2 ; SAPKK 2 ; SAPKK 3 ; SAPKK-2 ; SAPKK2 ; SAPKK3 ; Stress activated protein kinase kinase 2 ; Stress activated protein kinase kinase 3 ; Stress-activated protein kinase kinase 2.
背景
This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq, Jul 2008],
功能
Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Probably activated by dual phosphorylation on Ser-207 and Thr-211.,Function:Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.,induction:Strongly activated by UV, anisomycin, and osmotic shock but not by phorbol esters, NGF or EGF.,PTM:Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.,PTM:Weakly autophosphorylated.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with Yersinia yopJ.,tissue specificity:Isoform 2 is only expressed in skeletal muscle. Isoform 1, on the other hand, is found in skeletal muscle, heart, and in lesser extent in liver or pancreas.,
