蛋白名称
Wiskott-Aldrich syndrome protein family member 1
组成
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
来源
Monoclonal, Rabbit,IgG
稀释比例
IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
其他名称
WASF1 ; KIAA0269 ; SCAR1 ; WAVE1 ; Wiskott-Aldrich syndrome protein family member 1 ; WASP family protein member 1 ; Protein WAVE-1 ; Verprolin homology domain-containing protein 1
背景
The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],
功能
Domain:Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.,Function:Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton.,similarity:Belongs to the SCAR/WAVE family.,similarity:Contains 1 WH2 domain.,subcellular location:Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.,subunit:Component of the WAVE1 complex composed of ABI2, CYFIP2, C3orf10/HSPC300, NCKAP1 and WASF1/WAVE1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1 (By similarity). Binds actin and the Arp2/3 complex. Interacts with BAIAP2.,tissue specificity:Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.,
