基因名称(Gene Name)
Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
特异性
The antibody detects C-terminal, internal, and N-terminal Flag-tag fusion proteins.
组成
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
来源
Monoclonal, Rabbit,IgG
稀释比例
WB 1:10000-1:50000; ELISA 1:20000-50000; IF 1:100-300; IP 1:50-1:200,
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
背景
The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
