组成
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
来源
Monoclonal, Rabbit,IgG
稀释比例
IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
其他名称
SPP1 ; BNSP ; OPN ; PSEC0156 ; Osteopontin ; Bone sialoprotein 1 ; Nephropontin ; Secreted phosphoprotein 1 ; SPP-1 ; Urinary stone protein ; Uropontin
背景
secreted phosphoprotein 1(SPP1) Homo sapiens The protein encoded by this gene is involved inThe attachment of osteoclasts toThe mineralized bone matrix.The encoded protein is secreted and binds hydroxyapatite with high affinity.The osteoclast vitronectin receptor is found inThe cell membrane and may be involved inThe binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],
功能
Disease:This protein plays a principal role in urinary stone formation as the stone matrix.,Function:Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.,Function:Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.,online information:Osteopontin entry,PTM:Extensively phosphorylated on clustered serine residues.,PTM:N- and O-glycosylated.,similarity:Belongs to the osteopontin family.,subunit:Ligand for integrin alpha-V/beta-3.,
