组成
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
背景
This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016],
功能
Alternative products:Additional isoforms seem to exist,Catalytic activity:S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.,Caution:It is uncertain whether Met-1 or Met-21 is the initiator methionine.,Caution:PubMed:11707778 reported that while NG36 and G9a were originally thought to derive from two separate genes, all G9A transcripts also contain the in frame coding sequence of NG36.,Domain:The SET domain mediates interaction with WIZ.,Function:Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also methylates histone H1.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.,similarity:Contains 1 post-SET domain.,similarity:Contains 1 pre-SET domain.,similarity:Contains 1 SET domain.,similarity:Contains 7 ANK repeats.,subcellular location:Associates with euchromatic regions. Does not associate with heterochromatin.,subunit:Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with GFI1B, WIZ and EHMT1.,tissue specificity:Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.,
