基因名称(Gene Name)
QTRT1 TGT TGUT
蛋白名称
Queuine tRNA-ribosyltransferase (Guanine insertion enzyme) (tRNA-guanine transglycosylase)
免疫原
Synthesized peptide derived from human QTRT1. AA range:283-353
特异性
This antibody detects endogenous levels of QTRT1 at Human, Mouse,Rat
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
功能
Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU (N) anticodons (tRNA-Asp , -Asn , -His and -Tyr) , resulting in the hypermodified nucleoside queuosine (7- ( ( (4 ,5-cis-dihydroxy-2-cyclopenten-1-yl) amino) methyl) -7-deazaguanosine) . Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA , forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine , allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity) .
