背景
Puromycin is a tyrosyl-tRNA mimic that blocks translation by labeling and releasing
elongating polypeptide chains from translating ribosomes. Puromycin has been used in
molecular biology research for decades as a translation inhibitor.More recently, in vivo
puromycylation assays have become popular tools for localizing translating ribosomes in
cells. These assays often use elongation inhibitors to purportedly inhibit the release of
puromycin-labeled nascent peptides from ribosomes.
