组成
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
稀释比例
WB 1:2000-1:10000; IF 1:100-500; ELISA 1:1000-5000; IP 1:50-1:200;
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
背景
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
