基因名称(Gene Name)
CLP1 HEAB
蛋白名称
Polyribonucleotide 5'-hydroxyl-kinase Clp1 (EC 2.7.1.78) (Polynucleotide kinase Clp1) (Pre-mRNA cleavage complex II protein Clp1)
免疫原
Synthesized peptide derived from human protein . at AA range: 50-130
特异性
CLP1 Monoclonal Antibody detects endogenous levels of protein.
组成
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
纯化工艺
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.
背景
This gene encodes a member of the Clp1 family. The encoded protein is a multifunctional kinase which is a component of the tRNA splicing endonuclease complex and a component of the pre-mRNA cleavage complex II. This protein is implicated in tRNA, mRNA, and siRNA maturation. Mutations in this gene are associated with pontocerebellar hypoplasia type 10 (PCH10). Alternatively splice transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014],
功能
catalytic activity:ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.,catalytic activity:ATP + 5'-dephospho-RNA = ADP + 5'-phospho-RNA.,cofactor:Magnesium, manganese or nickel.,function:Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage compl
