免疫原
The antiserum was produced against synthesized peptide derived from human Dematin. AA range:356-405
特异性
Dematin Monoclonal Antibody detects endogenous levels of Dematin protein.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
Western Blot: 1/500 - 1/2000
纯化工艺
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.
其他名称
EPB49; DMT; Dematin; Erythrocyte membrane protein band 4.9
背景
The protein encoded by this gene is an actin binding and bundling protein that plays a structural role in erythrocytes, by stabilizing and attaching the spectrin/actin cytoskeleton to the erythrocyte membrane in a phosphorylation-dependent manner. This protein contains a core domain in the N-terminus, and a headpiece domain in the C-terminus that binds F-actin. When purified from erythrocytes, this protein exists as a trimer composed of two 48 kDa polypeptides and a 52 kDa polypeptide. The different subunits arise from alternative splicing in the 3' coding region, where the headpiece domain is located. Disruption of this gene has been correlated with the autosomal dominant Marie Unna hereditary hypotrichosis disease, while loss of heterozygosity of this gene is thought to play a role in prostate cancer progression. Alternative splicing results in multiple transcript variants encoding di
功能
domain:Consists of a large core fragment, the amino-terminal portion, and a small headpiece, the C-terminal portion. The headpiece can bind but cannot bundle actin filaments.,domain:Contains at least two actin-binding sites, one in the headpiece domain and one in the amino-terminal portion.,function:Actin-bundling protein. May function in mitogen-activated protein kinase pathway.,PTM:Actin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase.,PTM:The N-terminus is blocked.,similarity:Belongs to the villin/gelsolin family.,similarity:Contains 1 HP (headpiece) domain.,subunit:Exists in solution as a trimer of two short isoforms and one long isoform linked by disulfide bonds (Probable). Interacts with RASGRF2.,tissue specificity:Heart, brain, lung, skeletal muscle, and kidney.,
