蛋白名称
Crossover junction endonuclease EME1
免疫原
Synthesized peptide derived from Eme1 . at AA range: 250-330
特异性
Eme1 Monoclonal Antibody detects endogenous levels of Eme1 protein.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
稀释比例
Western Blot: 1/500 - 1/2000
纯化工艺
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.
其他名称
EME1; MMS4; Crossover junction endonuclease EME1; MMS4 homolog; hMMS4
背景
This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009],
功能
cofactor:Magnesium.,function:Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.,similarity:Belongs to the EME1/MMS4 family.,subcellular location:Recruited to regions of DNA damage in S-phase cells.,subunit:May self-associate. Interacts with MUS81. Interacts with ERCC4 and FANCM.,
