蛋白名称
Myosin regulatory light polypeptide 9
免疫原
The antiserum was produced against synthesized peptide derived from human Myosin regulatory light chain 2 around the phosphorylation site of Ser18. AA range:3-52
特异性
Phospho-MRLC2 (S20) Polyclonal Antibody detects endogenous levels of MRLC2 protein only when phosphorylated at S20.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):ATsNV
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
来源
Polyclonal, Rabbit,IgG
稀释比例
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.
Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
其他名称
MYL9;Myosin regulatory light polypeptide 9;MYL9 ;
MLC2 ;
MRLC1 ;
MYRL2 ;
Myosin regulatory light polypeptide 9 ;
20 kDa myosin light chain ;
LC20 ;
MLC-2C ;
Myosin RLC ;
Myosin regulatory light chain 2 ;
smooth muscle isoform ;
Myosin regulatory light chain 9 ;
Myosin regulatory light chain MRL
背景
Myosin , a structural component of muscle , consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq , Jul 2008] ,
功能
Function:Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis , receptor capping , and cell locomotion. ,miscellaneous:This chain binds calcium. ,PTM:Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. ,similarity:Contains 3 EF-hand domains. ,subunit:Myosin is an hexamer of 2 heavy chains and 4 light chains. ,tissue specificity:Smooth muscle tissues and in some , but not all , nonmuscle cells. ,
