免疫原
Synthesized peptide derived from human Tyrosinase AA range: 250-350
特异性
This antibody detects endogenous levels of Tyrosinase protein.
组成
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
稀释比例
IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000
Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
纯化工艺
Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
其他名称
TYR;Tyrosinase;ATN ;
CMM8 ;
LB24 AB ;
LB24-AB ;
Monophenol monooxygenase ;
OCA1 ;
OCA1A ;
OCAIA ;
Oculocutaneous albinism IA ;
SHEP3 ;
SK29 AB ;
SK29-AB ;
Tumor rejection antigen AB ;
TYR ;
TYRO_HUMAN ;
tyrosinase ;
oculocutaneous albinism IA ;
Tyrosinase
背景
tyrosinase (TYR) Homo sapiens The enzyme encoded by this gene catalyzes the first 2 steps , and at least 1 subsequent step , in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities , and requires copper for function. Mutations in this gene result in oculocutaneous albinism , and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq , Oct 2008] ,
功能
Catalytic activity:L-tyrosine + L-dopa + O (2) = L-dopa + dopaquinone + H (2) O. ,cofactor:Binds 2 copper ions per subunit. ,Disease:Defects in TYR are the cause of oculocutaneous albinism type I temperature-sensitive (OCA-ITS) [MIM:606952]. OCA-ITS patients have white axillary and scalp hair and pigmented arm and leg hair. ,Disease:Defects in TYR are the cause of oculocutaneous albinism type IA (OCA-IA) [MIM:203100]. OCA-I , also known as tyrosinase negative oculocutaneous albinism , is an autosomal recessive disorder characterized by absence of pigment in hair , skin and eyes. OCA-I is divided into 2 types: type IA , characterized by complete lack of tyrosinase activity due to production of an inactive enzyme , and type IB characterized by reduced activity of tyrosinase. OCA-IA patients presents with the life-long absence of melanin pigment after birth and manifest increased sensitivity to ultraviolet radiation and to predisposition to skin cancer. ,Disease:Defects in TYR are the cause of oculocutaneous albinism type IB (OCA-IB) [MIM:606952]; also known as albinism yellow mutant type. OCA-IB patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. ,Function:This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA , DOPA to DOPA-quinone and possibly 5 ,6-dihydroxyindole to indole-5 ,6 quinone. ,induction:Increased expression after UV-B radiation. ,online information:Retina International's Scientific Newsletter ,online information:Snowy stardom - Issue 49 of August 2004 ,online information:TYR mutations ,online information:Tyrosinase entry ,polymorphism:Compound heterozygosity for the R402Q polymorphism and a mutant allele of TYR is a common cause of autosomal recessive ocular albinism. The R402Q polymorphism is also found in Waardenburg syndrome type II with ocular albinism (WS2-OA) in association with a deletion in the MITF gene. ,polymorphism:Genetic variations in TYR are associated with skin/hair/eye pigmentation type 3 (SHEP3) [MIM:601800]. Hair , eye and skin pigmentation are among the most visible examples of human phenotypic variation , with a broad normal range that is subject to substantial geographic stratification. In the case of skin , individuals tend to have lighter pigmentation with increasing distance from the equator. By contrast , the majority of variation in human eye and hair color is found among individuals of European ancestry , with most other human populations fixed for brown eyes and black hair. ,similarity:Belongs to the tyrosinase family. ,
