应用范围
WB, IHC, IF, FC, ELISA
免疫原
Purified recombinant fragment of human N-cadherin expressed in E. Coli.
特异性
N-cadherin Monoclonal Antibody detects endogenous levels of N-cadherin protein.
组成
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
稀释比例
IHC 1:200-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. Note:For IHC,wesuggest antigen retrieval with TE buffer pH 9.0
纯化工艺
Affinity purification
其他名称
CDH2; CDHN; NCAD; Cadherin-2; CDw325; Neural cadherin; N-cadherin; CD antigen CD325
背景
This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015],
功能
function:Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism.,similarity:Contains 5 cadherin domains.,subunit:Interacts with CDCP1.,
