免疫原
The antiserum was produced against synthesized peptide derived from human SGOL1. AA range:271-320
特异性
Sgo1 Polyclonal Antibody detects endogenous levels of Sgo1 protein.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
来源
Polyclonal, Rabbit,IgG
稀释比例
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
其他名称
SGOL1; SGO1; Shugoshin-like 1; hSgo1; Serologically defined breast cancer antigen NY-BR-85
背景
The protein encoded by this gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibro
功能
developmental stage:Appears in prophase cells and remains present until metaphase. Strongly decreases at the onset of anaphase and completely disapears at telophase. Not present in interphase cells (at protein level).,domain:The D-box (destruction box) mediates the interaction with APC proteins, and may act as a recognition signal for degradation via the ubiquitin-proteasome pathway.,function:Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase.,miscellaneous:Strongly overexpressed in 90% of breast cancers tested.,PTM:Ubiquitinated by the ana
