蛋白名称
Serine/threonine-protein kinase PLK1
免疫原
The antiserum was produced against synthesized peptide derived from human PLK1. AA range:176-225
特异性
Plk Polyclonal Antibody detects endogenous levels of Plk protein.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
来源
Polyclonal, Rabbit,IgG
稀释比例
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
其他名称
PLK1; PLK; Serine/threonine-protein kinase PLK1; Polo-like kinase 1; PLK-1; Serine/threonine-protein kinase 13; STPK13
背景
The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015],
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,developmental stage:Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.,enzyme regulation:Activated by serine and threonine phosphorylation.,function:Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.,induction:By growth-stimulating agents.,PTM:Autophosphorylation and phosphorylation of Ser-137 are not significant events during activation of PLK1 in M phase.,PTM:Catalytic activity is enhanced by phosphorylation of Thr-210 and/or S
