蛋白名称
Lymphokine-activated killer T-cell-originated protein kinase
免疫原
The antiserum was produced against synthesized peptide derived from human PBK/TOPK around the phosphorylation site of Thr9. AA range:1-50
特异性
Phospho-PBK (T9) Polyclonal Antibody detects endogenous levels of PBK protein only when phosphorylated at T9.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
来源
Polyclonal, Rabbit,IgG
稀释比例
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
其他名称
PBK; TOPK; Lymphokine-activated killer T-cell-originated protein kinase; Cancer/testis antigen 84; CT84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinas
背景
PDZ binding kinase(PBK) Homo sapiens This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by phosphorylation.,function:Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.,PTM:Phosphorylated; in a cell-cycle dependent manner at mitosis.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with DLG1 and TP53.,tissue specificity:Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.,
