反应种属
Human;Mouse;Rat;Dog;Pig;Rabbit
基因名称(Gene Name)
CKMT1A/CKMT1B
蛋白名称
Creatine kinase U-type mitochondrial
免疫原
Purified recombinant human uMtCK protein fragments expressed in E.coli.
特异性
uMtCK Monoclonal Antibody detects endogenous levels of uMtCK protein.
组成
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
稀释比例
Western Blot: 1/1000 - 1/2000. Not yet tested in other applications.
纯化工艺
Affinity purification
其他名称
CKMT1A; CKMT; CKMT1B; CKMT; Creatine kinase U-type; mitochondrial; Acidic-type mitochondrial creatine kinase; Mia-CK; Ubiquitous mitochondrial creatine kinase; U-MtCK
背景
Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mi
功能
catalytic activity:ATP + creatine = ADP + phosphocreatine.,function:Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.,miscellaneous:Mitochondrial creatine kinase binds cardiolipin.,similarity:Belongs to the ATP:guanido phosphotransferase family.,subunit:Exists as an octamer composed of four MTCK homodimers.,
