反应种属
C. Elegans;Human;Mouse;Rat
基因名称(Gene Name)
C. Elegans β-actin
免疫原
Synthesized peptide derived from C. Elegans β-actin
特异性
This antibody detects endogenous levels of C. Elegans β-actin
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.07% sodium azide.
稀释比例
IHC-p1:100-500 ,WB 1:5000-20000
纯化工艺
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
背景
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.
