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UHRF1(N-term) mouse mAb

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货号: YP-Ab-02354
促销价：

产品介绍

反应种属
Human;Mouse
应用范围
WB
抗体类型
单克隆抗体
基因名称(Gene Name)
uhrf1
蛋白名称
分子量（DA）
90kD
免疫原
Purified recombinant human UHRF1 protein fragments expressed in E.coil.
特异性
This antibody detects endogenous levels of UHRF1 and does not cross-react with related proteins
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
来源
Monoclonal, Mouse
稀释比例
wb 1:1000
纯化工艺
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
浓度
1 mg/ml
储存
-20°C/1 year
其他名称
Ac2-121;AL022808;E3 ubiquitin-protein ligase UHRF1;EC 6.3.2.-;FLJ21925;Hnp95 Huhrf1; HuNp95;ICBP90;Inverted CCAAT box binding protein of 90 kDa;Inverted CCAAT box binding protein, 90-Kd;Inverted CCAAT box-binding protein of 90 kDa;Liver regeneration-related protein LRRG126;MGC138707;NP95;Nuclear phosphoprotein, 95-KD;Nuclear protein 95;Nuclear zinc finger protein Np95;RING finger protein 106;RNF106;Transcription factor ICBP90; Ubiquitin like containing PHD and RING finger domains protein 1;Ubiquitin like PHD and RING finger domain containing protein 1;Ubiquitin-like PHD and RING finger domain-containing protein 1;Ubiquitin-like protein containing PHD and RING finger domains 1; Ubiquitin-like with PHD and ring finger domains 1;Ubiquitin-like, containing PHD and RING finger domains, 1;Ubiquitin-like-containing PHD and RING finger domains protein 1;UHRF1;UHRF1_HUMAN.
背景
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014],
功能
developmental stage:Expressed in fetal thymus, liver and kidney.,domain:The RING finger is required for ubiquitin ligase activity.,domain:The YDG domain mediates the interaction with histone H3.,function:Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.,induction:Up-regulated in proliferating cells, and down-regulated in quiescent cells. Down-regulated upon adriamycin-induced DNA damage, in a TP53/p53 and CDKN1A-dependent way. Induced by E2F1 transcription factor.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.,PTM:Ubiquitinated; which leads to proteasomal degrad

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优品生物的抗体，可以回收利用几次？

首先，一般抗体不推荐客户回收利用，抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，优品生物也做过一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定。
注：我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存，效价高的抗体可至少保存1周，至少重复利用3次。

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优品生物作为国产抗体品牌的优势？

优品生物是国产品牌，通过聚焦抗体与酶核心技术，致力于打破国际技术的垄断，我们拥有包括兔多克隆抗体、小鼠单克隆抗体、兔单克隆抗体的生产研发平台，同时也有包括WB,IP在内的检测平台，我们对每一支自产的抗体进行了严格的检测。当然，我们部分直销地区也可以帮客户代购进口品牌的产品。同时也有抗体定制服务。优品生物l抗体优势：1，严自检，保质量；2产品多，指标全；3，价格低，货期短。

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优品生物抗体成分？

优品生物抗体成分在平时工作当中，常会有客户咨询我们的抗体用的什么buffer进行保存，一般来说，我们的buffer的成分是：PBS含0.03%的proclin300、0.05%牛血清白蛋白、50%甘油；也有一些是PBS含0.03%的proclin300，50%甘油。防腐剂 Proclin 300活性成分主要是2-甲基-4-异噻唑啉-3-酮（MCI）和5-氯-2-甲基-4-异噻唑啉-3-酮（CMCI）。ProClin生物灭活剂能够迅速穿透细胞膜，抑制对细胞呼吸至关重要的特定酶，因此一接触微生物有机体就会立即抑制细胞活性。ProClin的多个特定毒性位点可以防止微生物产生高水平的耐药性。

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