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MyoD (phospho Ser200) Polyclonal Antibody

货号: YP-Ab-01296
促销价:

产品介绍

反应种属
Human;Mouse;Rat
应用范围
WB;IHC;IF;ELISA
抗体类型
多克隆抗体
基因名称(Gene Name)
MYOD1
蛋白名称
Myoblast determination protein 1
分子量(DA)
34kD
免疫原
The antiserum was produced against synthesized peptide derived from human MYOD around the phosphorylation site of Ser200. AA range:171-220
特异性
Phospho-MyoD (S200) Polyclonal Antibody detects endogenous levels of MyoD protein only when phosphorylated at S200.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
来源
Polyclonal, Rabbit,IgG
稀释比例
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/5000.. IF 1:50-200
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
浓度
1 mg/ml
储存
-20°C/1 year
其他名称
MYOD1; BHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3
背景
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008],
功能
function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.,online information:MyoD entry,PTM:Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.,PTM:Ubiquitinated on the N-terminus; which is required for proteasomal degradation.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1.,

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