反应种属
Human;Mouse;Rat;Monkey
蛋白名称
Transcription factor Sp1
免疫原
The antiserum was produced against synthesized peptide derived from human SP1 around the phosphorylation site of Thr739. AA range:706-755
特异性
Phospho-Sp1 (T739) Polyclonal Antibody detects endogenous levels of Sp1 protein only when phosphorylated at T739.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
来源
Polyclonal, Rabbit,IgG
稀释比例
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/5000.. IF 1:50-200
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
其他名称
SP1; TSFP1; Transcription factor Sp1
背景
The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014],
功能
function:Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter.,PTM:O-glycosylated; contains N-acetylglucosamine side chains.,similarity:Belongs to the Sp1 C2H2-type zinc-finger protein family.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr. Interacts with SV40 VP2/3 proteins. Interacts with SV40 major capsid protein VP1; this interaction leads to a cooperativity between the two proteins in DNA binding.,
