蛋白名称
Retinoblastoma-associated protein
免疫原
Purified recombinant fragment of human Rb expressed in E. Coli.
特异性
Rb Monoclonal Antibody detects endogenous levels of Rb protein.
组成
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
稀释比例
IHC: 1/200 - 1/1000. ELISA: 1/10000.. IF 1:50-200
纯化工艺
Affinity purification
其他名称
RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
背景
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008],
功能
disease:Defects in RB1 are a cause of bladder cancer [MIM:109800].,disease:Defects in RB1 are a cause of osteogenic sarcoma [MIM:259500].,disease:Defects in RB1 are the cause of childhood cancer retinoblastoma (RB) [MIM:180200]. RB is a congenital malignant tumor that arises from the nuclear layers of the retina. It occurs in about 1:20'000 live births and represents about 2% of childhood malignancies. It is bilateral in about 30% of cases. Although most RB appear sporadically, about 20% are transmitted as an autosomal dominant trait with incomplete penetrance. The diagnosis is usually made before the age of 2 years when strabismus or a gray to yellow reflex from pupil ("cat eye") is investigated.,function:Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts
